The Impact of Mustard Oil (Brassica Juncea) Ingestion on Thyroid Gland Hormones in Experimental Rats

Document Type : Original Article


1 nutrition and food science faculty of home economic helwan university

2 Nutrition and Food Science Dept., Faculty of Home Economics, Helwan University,Cairo,Egypt.


The present study was conducted to investigate the impact of mustard oil ingestion on thyroid hormones in experimental rats .Forty-two adult male albino rats (180± 5 g) were divided into 7 groups. Group (1) (-ve control) rats were fed on basal diet. Groups 2-4 were rendered hypothyroidism (injected with PTU, ip), where G2 fed on basal diet and G 3& 4 were given mustard oil at two levels (1&2 ml/Kg Bwt.), respectively. Groups 5-7 were rendered hyperthyroidism (injected with L-thyroxin, ip), where G5 was fed on basal diet and G6& 7 were given mustard oil at the same two levels, respectively. At the end of the experimental period (8 weeks), rats were sacrificed and blood samples were collected for serum. Thyroid hormones, liver and kidney functions were determined. Results indicated that, hypothyroid rats showed significant reduction in FT3 and FT4 levels, however, TSH level was significantly increased compared to the healthy group. Ingestion of mustard oil at the two levels of intake improve in hypothyroid status as related to significant increase in serum concentration of FT3 and FT4 and significant reduction of the level of TSH. While hyperthyroid rats showed significant increase in FT4 and FT3 levels. TSH level was significantly increased. Ingestion of mustard oil at the two levels caused lower in hyperthyroid status as related to significant decreased in the concentration of FT4 and FT3 and significant increase of the level of TSH. It was also observed that ingestion of mustard oil improve kidney function by reducing creatinine and urea concentrations in serum in the hypo and hyperthyroidism. Resuts revealed that mustard oil had beneficial effect on AST activity in rats with hypo and hyperthyroidism. Our results concluded that mustard oil ingestion improves thyroid hormones, liver and kidney functions. Thus, it could be recommended for patients with thyroid dysfunctions.